Enzymes accelerate, or catalyze, chemical reactions, and they are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.
Histo/Zyme
Supplier: SIGMA ALDRICH MICROSCOPY
Histo/Zyme is a stabilized proteolytic enzyme in buffered solution, used to unmask immunoreactive sites altered by fixation and/or the embedding process. Treatment with a proteolytic enzyme in immunohistochemistry (IHC) is required to digest excess linkages and expose the antigen. The use of Histo/Zyme results in superior staining over traditionally used enzyme pretreatments, such as pepsin and trypsin. Moreover, Histo/Zyme requires only a brief incubation at room temperature. Performing antigen recovery with Histo/Zyme will allow you to dilute the primary antibody 5 to 10 fold further and achieve optimal staining.
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Pronase E (from Streptomyces griseus)
Supplier: Merck
Pronase E is a mixture of proteolytic enzymes that is obtained from Streptomyces griseus. It can be used in the in diagnostics in histopathology as it hydrolyses proteins into individual amino acids.
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Tritirachium album proteinase K
Supplier: Merck
Proteinase K (from tritirachium album) solution in tris/HCl for molecular biology.
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Oxidase, Bactident®, Millipore®
Supplier: Merck
Oxidase used for the detection of cytochrome oxidase in microorganisms.
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VWR® Trichoderma viride Cellulase
Supplier: VWR Chemicals
10,000 units is that quantity of enzyme which will degrade 2 cm² of cellulose filter paper (17 mg) in 1 minute in 0,1 M acetate buffer at pH 4,0.
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Bovine Thrombin (from Plasma)
Supplier: SIGMA ALDRICH MICROSCOPY
Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
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Aldehyde dehydrogenase (from Yeast)
Supplier: Merck
Aldehyde dehydrogenase (from Yeast)
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VWR®, Pepsin 1:10000
Supplier: VWR Chemicals
Pepsin is a proteolytic enzyme product derived from porcine stomach linings.
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VWR® Native Bovine Trypsin (from Pancreas)
Supplier: VWR Chemicals
From bovine pancreas.
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VWR®, Aspergillus Glucose oxidase
Supplier: VWR Chemicals
Beta-D-glucose oxidase with high purity grade and storage in frozen condition.
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VWR®, Bovine Ribonuclease A (from Pancreas)
Supplier: VWR Chemicals
Ribonuclease A is an endoribonuclease that efficiently hydrolyses RNA from tissue or bacterial cell cultures.
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VWR®, Cod Uracil-DNA glycosylase
Supplier: VWR Chemicals
Uracil-DNA glycosylase (UNG), cod is a thermolabile recombinant enzyme produced in E. coli (ung-) using a modified ung gene derived from Atlantic cod. It degrades uracil-containing single- and double-stranded DNA, but not RNA or thymidine-containing DNA, by hydrolysing the N-glycosidic bond between deoxyribose sugar and the base in uracil. This generates alkaline-sensitive apyramidinic sites in the DNA that will be cleaved upon a combination of alkaline conditions and high temperature.
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VWR®, Pronase E
Supplier: VWR Chemicals
Off-white to light brown powder from Streptomyces griseus.
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VWR®, HL-dsDNase
Supplier: VWR Chemicals
VWR® Life Science's DNase, Double-Strand Specific, Heat-Labile is a recombinant endonuclease that cleaves phosphodiester bonds in DNA to yield 2 to 8 bp oligonucleotides with 5’-phosphate and 3’-hydroxyl termini.
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Pig Pepsin (from Stomach)
Supplier: SIGMA ALDRICH MICROSCOPY
Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. Pepsin digestion of FFPE tissue sections improves accessibility of antibodies to tissue antigens. Ready to use pepsin reagent, antigen retriever has been used for antigen unmasking in immunohistochemistry.
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J.T.Baker® Endonuclease
Supplier: Avantor
J.T.Baker® Endonuclease Ultrapure Bioreagent is designed for the degradation of both single stranded and double stranded DNA and RNA. J.T.Baker® Endonuclease Ultrapure Bioreagent is used to ensure host cell DNA impurities are removed; driving process efficiency by lowering viscosity and preventing aggregation. J.T.Baker® Endonuclease is an enzyme based upon the native endonuclease of Serratia marcescens, enabling rapid clearance of residual DNA and RNA during the production and purification of both recombinant proteins and viral vectors. Non-animal origin. The purity of materials in non-negotiable. J.T.Baker® Endonuclease Ultrapure Bioreagent acts to degrade and eliminate extraneous genetic material, ensuring the pristine quality of your final product.
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Recombinant human lipoprotein lipase/LPL protein, CF
Supplier: Novus Biologicals
Lipo protein lipase (LPL) is a rate-limiting enzyme responsible for the hydrolysis of triglycerides.
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Recombinant Human DNA topoisomerase I (from Insect cells (Sf9))
Supplier: US Biological
DNA topoisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription.
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J.T.Baker® Endonuclease
Supplier: Avantor
J.T.Baker® Endonuclease Biotech Reagent meets the strictest cGMP standards and is designed for the degradation of both single stranded and double stranded DNA and RNA. J.T.Baker® Endonuclease Biotech Reagent is used to ensure host cell DNA impurities are removed; driving process efficiency by lowering viscosity and preventing aggregation. J.T.Baker® Endonuclease Biotech Reagent is an enzyme based upon the native endonuclease of Serratia marcescens, enabling rapid clearance of residual DNA and RNA during the production and purification of both recombinant proteins and viral vectors. Non-animal origin. Absence of proteolytic activity. The purity of materials in non-negotiable. J.T.Baker® Endonuclease Biotech Reagent acts to degrade and eliminate extraneous genetic material, ensuring the pristine quality of your final product.
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Tritirachium album proteinase K
Supplier: Rockland Immunochemicals
Tritirachium album proteinase K