E.Z.N.A.® Circulating DNA kit provides a rapid and easy method for the isolation of circulating DNA from plasma, serum, and other cell-free body fluids.
- High sensitivity
- Easy processing
The kit allows single or multiple, simultaneous processing of samples in under 120 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation, and precipitation with isopropanol or ethanol, are eliminated. DNA purified using the E.Z.N.A® Circulating DNA method is ready for applications such as PCR, circulating detection, and genotyping.
E.Z.N.A.® Circulating DNA kit uses the reversible nucleic acid-binding properties of HiBind® matrix, combined with the speed of mini-column spin technology. A specifically formulated buffer system allows circulating DNA to bind to the matrix. Samples are first lysed under denaturing conditions and then applied to the HiBind® DNA spin columns to which DNA binds, while cellular debris, haemoglobin, and other proteins are effectively washed away. High quality DNA is finally eluted in sterile deionised water or low salt buffer.