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Anti-GFP Mouse Monoclonal Antibody [clone: 4B10B2]

Lieferant: Thermo Scientific
MA5-15349 targets GFP in flow cytometry, immunohistochemistry, and Western blot applications and shows reactivity with Tag samples. The MA5-15349 immunogen is purified recombinant fragment of GFP expressed in E. Coli. MA5-15349 detects GFP which has a predicted molecular weight of approximately 27kDa. GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

Type: Primary
Antigen: GFP
Clonality: Monoclonal
Clone: 4B10B2
Conjugation: Unconjugated
Host: Mouse
Isotype: IgG2a
Reactivity: Tag

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100 ug 100 µG PIEAMA515349 Blocked
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Antigen symbol GFP
Antigen name Green Fluorescence Protein
Reaktivität Tag
Konjugation Unconjugated
Klonalität Monoclonal
Antikörper Typ Primary
Clone 4B10B2
Wirt Mouse
Isotyp IgG2a
Western Blot Yes
ImmunoChemistry Yes
ImmunoFluorescence Yes
Flow cytometry Yes
Antigen synonyms GFP2, eGFP, Turbo GFP, Green Fluoresecent Protein, GFP
Immunogen Purified recombinant fragment of GFP expressed in E. Coli.
Purification purified
Form Liquid
Storage Buffer PBS, 0.03% sodium azide
Storage temperature Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Transporttemperatur Ice
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