This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
This antiserum stains cholinergic neurons in guinea-pig and rabbit.
Application Information:
Immunohistochemistry/cytochemistry on 4% PFA or formalin fixed frozen sections; paraffin sections can be more difficult and require more extensive antigen recovery methods. 1:400-1:2000 depending up detection and incubation times<br>ELISA: direct antigen ELISA<br>This antiserum will superbly stain both cell bodies and nerve terminal, and works particularly well in enteric and peripheral neurons. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: ChAT
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope: porcine ChAT protein (aa: 167-188)
Host: Rabbit
Isotype: IgG
Reactivity: Guinea Pig, Mouse, Rat, Rabbit
