Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one 'a' subunit and one 'b' tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2 a and MAP2 b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites.
The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Application Information:
Immunohistochemistry (IHC) and Western Blotting (WB). A dilution of 1:5,000-1:10,000 is recommended for WB. A dilution of 1:500-1:1000 is recommended for IHC. The optimal dilution should be determined by the end user.
Type: Primary
Antigen: MAP2
Clonality: Monoclonal
Clone: 5H11
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity: Human, Rat, Mouse
