Bis-Tris Gel Buffer, 4× is used to prepare polyacrylamide gels for optimal separation of small- to medium-sized proteins under denaturing conditions.
- Faster run time-separate your proteins in less than 35 min
- Obtain high resolution of small- to medium-sized proteins
- Neutral pH of the gel minimises protein modifications
- Store cold
The neutral pH environment of the gel prepared with Bis-Tris Gel Buffer minimizes protein modifications. Bis-Tris Gel Buffer, 4× can be used to prepare both separating and stacking gels with your standard protocol for polyacrylamide gels preparation. MES-SDS or MOPS-SDS buffer is then used as the running buffer to separate your proteins on a Bis-Tris gel. When using MES-SDS buffer, separation of proteins on a 10% Bis-Tris gel can be accomplished in less than 35 min.