Lieferant: Enzo Life Sciences
ALEXBML-P303-0005EA 215 EUR
Proteine und Peptide
Proteinase substrate

Much more sensitive substrate for leukocyte proteinase 3 (PR-3, myeloblastin) (Kcat/km=1.06 x 106 M-1s-1, Km=63 µM) than MeOSuc-AAPV-pNA (Chromogenic Substrate) (Prod. No. BML-P213) (Kcat/km=1.06 x 103 M-1s-1, Km=470 µM). Also cleaved by neutrophil elastase (Kcat/km=9,2×106 M-1s-1, Km=2.2 µM), granzyme H, cathepsin G, chymotrypsin, and rat MCPI and II. Typical assay conditions: substrate concentration 88-250 µM; enzyme concentration 0.2-20 nM; buffer [100 mM HEPES pH 7,5, 500 mM NaCl, 10% DMSO]; with 170 µM either 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) or 4,4'-dithiobis(pyridine) (from 15 mM DMSO stocks); 25°C. Hydrolysis of substrate can be detected at 412 nm for DTNB (

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