Atto 647 NHS ester is a reagent for the covalent labeling of molecules with Atto 647 flourescent dye.
- λex 643 nm; λem 669 nm in 0,1 M phosphate pH 7,0
- Designed for high sensitivity applications, including single molecule detection
Atto 647 belongs to a new generation of fluorescent labels for the red spectral region. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, photostability, water solubility, and very little triplet formation.
Atto 647 is a zwitterionic dye with a net electrical charge of zero. Atto 647 is a pH sensitive product. While practically stable up to pH 8,5, it slowly degrades at higher pH. Atto labels have rigid structures that do not show any cis-trans-isomerisation. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
