Mature BDNF/proBDNF Combo Rapid ELISA Kit (BEK-2211/2237): Human, Mouse, RatLieferant: Biosensis
BSENBEK-2241EA 682 EURBSENBEK-2241
Mature BDNF/proBDNF Combo Rapid ELISA Kit (BEK-2211/2237): Human, Mouse, Rat
The Biosensis Mature BDNF/proBDNF Combo RapidTM enzyme-linked immunosorbent assay (ELISA) Kit combines individual, but complementary ELISA kits for the two most important BDNF isoforms: Mature BDNF (BSENBEK-2211) and full-length proBDNF (BSENBEK-2237).
Both kits are sandwich ELISAs, useful for the quantification of mature BDNF and proBDNF in less than 4 hours in cell culture supernatants, serum, plasma (citrate and EDTA), mouse and rat cell lysates and rat brain extracts only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Please refer to the most current kit protocols for BSENBEK-2211 (Mature BDNF RapidTM ELISA) and BSENBEK-2237 (proBDNF RapidTM ELISA), for specific use instructions for each substrate application, in particular blood samples. BSENBEK-2241 is the preferred Combo kit for human blood samples.
The Mature BDNF ELISA kit consists of a pre-coated mouse monoclonal anti-mature BDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The proBDNF ELISA kit consists of a pre-coated monoclonal anti-proBDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature BDNF or proBDNF present in samples and protein standards. A BDNF and proBDNF positive control (QC sample) is provided to assure consistent assay performance.
Both ELISA kits contain high quality protein calibrators. The Mature BDNF ELISA kit employs a recombinant E.coli-derived human mature BDNF standard approved by the World Health Organization (WHO, www.nibsc.org). The amino acid sequence of mature BDNF is identical for human, mouse, rat and a number of other species. This kit therefore is suitable to measure mature BDNF in all these species and uses the same antibodies and antigen. The proBDNF ELISA kit employs a recombinant mammalian, cleavage-resistant human proBDNF standard produced by Biosensis and validated against externally available proBDNF proteins. Due to a high degree of amino acid sequence homology, mouse and rat proBDNF can be quantified and expressed as human proBDNF equivalents. Internal Biosensis validation suggests that the use of the human standard provided in this kit will provide estimates that are identical, or close, to the actual levels of rat and mouse proBDNF present in rodent samples.
Note that accurate proBDNF quantification in human serum and plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit.
Lieferumfang: The ELISA kit box contains 2×96-well pre-coated strip plates (1×BDNF antibody, 1×proBDNF antibody), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Bitte beachten For research use only. Not for diagnostic or clinical purposes.
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