pBK-CMV vector used to express proteins in both E. coli and mammalian model systems.
- Constitutive expression in eukaryotic cells
- Inducible protein expression in prokaryotes
- Rescued from ZAP Express lambda vector
In mammalian cells, the expression is driven by the CMV promoter and is therefore constitutively expressed. In E. coli, the lac promoter drives high-level expression in the presence of the inducer IPTG, and allows minimal expression in the absence of inducer.
Allows both prokaryotic and eukaryotic expression.
Lieferumfang: Kit contains 20 μg pBK-CMV phagemid vector, 500 μl XL1-Blue MRF, and 1 ml R408 Interference-Resistance helper phage.
Bitte beachten For research use only. Not for use in diagnostic procedures.