Ligation sequencing kits

Lieferant: Oxford Nanopore Technologies
OXNTSQK-LSK109EA 569 EUR
OXNTSQK-LSK109 OXNTSQK-NBD114.96 OXNTSQK-LSK110 OXNTSQK-NBD112.96 OXNTSQK-NBD114.24 OXNTSQK-LSK114 OXNTSQK-LSK112
Ligation sequencing kits
Nukleinsäure-Reagenzien Next Generation Sequencing Reagenzien
Ligation Sequencing Kits enable ligation-based, PCR-free nanopore sequencing of native DNA.

  • Sequence native DNA — eliminate PCR bias and retain base modifications
  • High sequencing yields
  • Control over read length
  • Suitable for upstream processes such as size selection or whole-genome amplification

Ligation Sequencing Kits offer a flexible method of preparing sequencing libraries from dsDNA (e.g. gDNA, cDNA, or amplicons) for nanopore sequencing. A range of Ligation Sequencing Kits are available to suit your experimental requirements:

Ligation Sequencing Kit (SQK-LSK114) – the latest nanopore sequencing chemistry, optimised to achieve sequencing accuracies of over 99% (Q20+) when combined with R10.4.1 flow cells (FLO-MIN114). This kit is available in three formats: Ligation Sequencing Kit (SQK-LSK114) – for singleplex samples; Native Barcoding Kit 24 (SQK-NBD114.24) – standalone kit enabling PCR-free multiplexing of up to 24 samples per run; and Native Barcoding Kit 96 (SQK-NBD114.96) – standalone kit enabling PCR-free multiplexing of up to 96 samples per run. Note: These kits are compatible with R10.4.1 flow cells only.

Ligation Sequencing Kit (SQK-LSK110) – this product has been superseded by Ligation Sequencing Kit (SQK-LSK114). SQK-LSK110 is anticipated to be discontinued during 2023. Note: SQK-LSK110 is compatible with R9.4.1 flow cells only.

Ligation Sequencing Kit (SQK-LSK109) – this product has been superseded by Ligation Sequencing Kit (SQK-LSK114). SQK-LSK109 is anticipated to be discontinued during 2023, except where used in COVID-related projects, which will be supported indefinitely. Note: SQK-LSK109 is compatible with R9.4.1 flow cells only. The Ligation Sequencing Kit (SQK-LSK109) includes a Flow Cell Priming Kit.

The library preparation method is straightforward: DNA ends are repaired and dA-tailed using the NEBNext End Repair/dA-tailing module, and then sequencing adapters, supplied in the kit, are ligated onto the prepared ends. With no requirement for amplification, these kits eliminate potential PCR bias and preserve base modifications, which can be detected alongside the nucleotide sequence.

For highest data yields, we recommend starting with 100 to 200 fmol* of pure input DNA for singleplex samples. Starting with lower amounts of input material, or impure samples, can affect library preparation efficiency and can reduce sequencing throughput.
*50 to 100 fmol for Ligation Sequencing Kit (SQK-LSK110).

Ligation Sequencing Kits are compatible with upstream processes such as target enrichment by sequence capture, whole genome amplification (for applications where under 1 ng of sample is available) and size selection (for enrichment of specified fragment lengths, using the BluePippin, for example). When size selecting, we recommend increasing the amount of input used, as size selection can be a wasteful process.

PCR- and WGA-free workflows remove amplification bias and retain base modification information, which can be analysed using tools supported by Oxford Nanopore.

The Ligation Sequencing Kit (SQK-LSK114) and the Native Barcoding Kits (SQK-NBD114.24 / SQK-NBD114.96) are compatible with R10.4.1 Flow Cells only. Ligation Sequencing Kits (SQK-LSK110 and SQK-LSK109) are compatible with R9.4.1 Flow Cells only.

Bestellinformation: SQK-LSK109 and SQK-LSK110 are anticipated to be discontinued in 2023. SQK-LSK109 will be available indefinitely for COVID-related projects.

Lieferumfang: Kits are shipped at 2 to 8 °C in an environmentally-friendly temperature-controlled shipping box.

Upon receipt, please place the product in a long-term storage location at ‒20 °C.

Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

Bitte beachten Oxford Nanopore Technologies products are not intended for use for health assessment or to diagnose, treat, mitigate, cure, or prevent any disease or condition.
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Please note that Oxford Nanopore Technologies requires the acceptance of their Terms & Conditions prior to product order placement. If you select product(s) to order, the Terms and Conditions will be displayed in the shopping cart for your review and acceptance.

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