Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit

Lieferant: Thermo Scientific
PIER16184EA 1042 EUR
PIER16184 PIER16183
Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit
Tests Enzymtests
The Thermo Scientific Pierce Cypridina-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.

  • Simultaneous—concurrent, filter-based, wavelength-separated detection of two luciferase activities
  • Sensitive—measure bright blue Cypridina and red firefly luciferase activities in the same sample
  • Fast—no quenching step required, unlike in traditional sequential dual assays
  • Multiplex—capable of quantitating two cellular activities in the same sample(s)

Cypridina luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control. The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. The resulting luminescent signals are spectrally resolvable using filters. In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors.

Lieferumfang: Kit contains Cypridina-Firefly Dual Assay Buffer (5 or 50 ml); 100X Vargulin (50 or 500 ml); D-Luciferin (3 or 30 mg); and 2X Luciferase Cell Lysis Buffer (6 or 60 L).

Bitte beachten For Research Use Only. Not for use in diagnostic procedures.

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